Journal: bioRxiv
Article Title: Observing picomolar protein unfolding using resonance light scattering
doi: 10.1101/2024.11.20.624557
Figure Lengend Snippet: Melting point determination and detection of BSA at ultralow concentration without fluorescent dyes. BSA detection (A) and MP (B) at 1μM, BSA detection (C) and MP (D) at 1nM (69 ± 3°C), and BSA detection (E) and MP (F) at 1pM concentrations, the insert remarks the difference between blank and 1pM BSA concentartion. The excitation wavelength was at 295 nm and emission range was between 330 nm to 400 nm. The average melting point curve corresponding to BSA at 1pM (59.09 ± 1.28°C, After Correction: 61.79 °C) the insert shows the signal respect to the blank (F). Melting point of the BoNT-A (65°C) at 3.3 pM and HSA (48°C) at 2.8 nM in BOCOUTURE (G). Melting point of the HSA (52°C) at 2.8 nM of concentration determined by the standard calibration curve by using a commercial HSA-CSL sample (H). The thermal scans were in the optimal optical properties of the cuvettes. See SI for scans in the range 20 – 90 °C.
Article Snippet: Human Serum Albumin (HSA) (Lot: P100491951) was acquired from CSL Behring GmbH, Tyskland, Germany, and Dry Botulinum Toxin formulation (BOCOUTURE) (Lot: 139059) from Merz Pharmaceuticals GmbH, 60318 Frankfurt/Main Tyskland, Germany; both preparation was dissolved in NaCl 0.9% (Lot: 22452413) from B. Braun Melsungen AG 34209 Melsungen, Germany.
Techniques: Concentration Assay